한우에서 임신 초기 발현 차이 혈장 단백질의 단백질체학적 분석

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 34권 / 3호
ㆍ저자명 : 김평희, 권대진, 오건봉, 이휘철, 양병철, 임기순, 민관식, 윤종택, 진동일, 박수봉, 황성수

목차

ABSTRACT 서론 재료 및 방법 공시동물 호르몬 분석 혈장 단백질 추출 2차원 전기영통 질량분석을 위한 단백질 절편화 단백질 통정 Western Blot Analysis 통계분석 결과 고찰 인용문헌

영어 초록

This study was performed to comprehend the plasma proteins expressed specifically during early pregnancy in pregnant or non-pregnant Hanwoo using proteomic analysis technique. Plasma samples (0, 2, 3, 4, 7, and 11 weeks after AI) were obtained from pregnant (P, n=3) or non-pregnant (NP, n=4) Hanwoo, respectively. To evaluate proteins differentially expressed, 2-dimensional electrophoresis (2DE) was conducted. Normalized protein spots were selected for the significant expression variation deviated over two fold in its expression level between two groups. Molecular functions of the proteins were DNA binding, protein binding, hemoglobin binding, ferrochelatase and transporter activity and arylestera, respectively. According to western blotting, haptoglobin was specifically expressed only in NP group during early pregnancy; however, paraoxonase 1 was highly expressed in pregnant group. Based on these results, pregnancy was maintained successfully by the activation of specific plasma proteins associated with immune system and antioxidant regulation during early pregnancy in Hanwoo

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