Xylitol stimulates saliva secretion via muscarinic receptor signaling pathway

서지정보

ㆍ발행기관 : 대한구강생물학회 ㆍ수록지정보 : International Journal of Oral Biology / 44권 / 2호
ㆍ저자명 : Eunjoo Park, Hee Sam Na, Sunghee Jeong, Jin Chung

목차

Introduction
Materials and Methods
1. Cell culture
2. Reagents
3. Subjects
4. Measurement of intracellular calcium concentration
5. Real-time quantitative reverse-transcriptasepolymerase chain reaction (qRT-PCR)
6. Western blotting
7. Membrane protein purification
8. Immunostaining
9. Statistics
Results
1. Xylitol stimulates salivary secretion in both healthyand dry mouth patients
2. Xylitol increases intracellular calcium concentrationin HSG cells via M3R signaling pathway
3. Xylitol induces M3R expression in HSG cells viastimulation of M3R signaling pathway
4. Xylitol induces AQP5 expression via M3R signalingpathway
Discussion
References

영어 초록

Xylitol is well-known to have an anti-caries effect by inhibiting the replication of cariogenic bacteria. In addition, xylitol enhances saliva secretion. However, the precise molecular mechanism of xylitol on saliva secretion is yet to be elucidated. Thus, in this study, we aimed to investigate the stimulatory effect of xylitol on saliva secretion and to further evaluate the involvement of xylitol in muscarinic type 3 receptor (M3R) signaling. For determining these effects, we measured the saliva flow rate following xylitol treatment in healthy individuals and patients with dry mouth. We further tested the effects of xylitol on M3R signaling in human salivary gland (HSG) cells using realtime quantitative reverse-transcriptase polymerase chain reaction, immunoblotting, and immunostaining. Xylitol candy significantly increased the salivary flow rate and intracellular calcium release in HSG cells via the M3R signaling pathway. In addition, the expressions of M3R and aquaporin 5 were induced by xylitol treatment. Lastly, we investigated the distribution of M3R and aquaporin 5 in HSG cells. Xylitol was found to activate M3R, thereby inducing increases in Ca2+ concentration. Stimulation of the muscarinic receptor induced by xylitol activated the internalization of M3R and subsequent trafficking of aquaporin 5. Taken together, these findings suggest a molecular mechanism for secretory effects of xylitol on salivary epithelial cells.

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