Induction of Prostaglandin E2 by Porphyromonas gingivalis in Human Dental Pulp Cells

서지정보

ㆍ발행기관 : 대한구강생물학회 ㆍ수록지정보 : International Journal of Oral Biology / 42권 / 4호
ㆍ저자명 : So-Hee Kim, Yun-Woong Paek, In-Chol Kang

목차

Introduction
Materials and Methods
Reagents
Bacterial culture
HDPCs culture
RT-PCR
ELISA
Western blot
Statistical analysis
Results
Induction of COX-2 mRNA expression
Production of PGE2
Activation of MAPKs and NF-κB pathways
Effects of various signaling inhibitors
Discussion
Acknowledgments
Conflict of interest
References

영어 초록

Cyclooxygenase-2 (COX-2)-mediated prostaglandin E2 (PGE2) plays a key role in development and progression of inflammatory responses and Porphyromonas gingivalis is a common endodontic pathogen. In this study, we investigated induction of COX-2 and PGE2 by P. gingivalis in human dental pulp cells (HDPCs). P. gingivalis increased expression of COX-2, but not that of COX-1. Increased levels of PGE2 were released from P. gingivalis-infected HDPCs and this PGE2 increase was blocked by celecoxib, a selective COX-2 inhibitor. P. gingivalis activated all three types of mitogen-activated protein kinases (MAPKs). P. gingivalis-induced activation of nuclear factor-κB (NF-κB) was demonstrated by the results of phosphorylation of NF-κ B p65 and degradation of inhibitor of κB-α (IκB-α). Pharmacological inhibition of each of the three types of MAPKs and NF-κB substantially attenuated P. gingivalisinduced PGE2 production. These results suggest that P. gingivalis should promote endodontic inflammation by stimulating dental pulp cells to produce PGE2.

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