Luteolin Induces Apoptosis via Mitochondrial Pathway and Inhibits Invasion and Migration of Oral Squamous Cell Carcinoma by Suppressing Epithelial-Mesenchymal Transition Induced Transcription Factors
(주)코리아스칼라
- 최초 등록일
- 2023.04.03
- 최종 저작일
- 2018.06
- 8페이지/ 어도비 PDF
- 가격 4,000원
* 본 문서는 배포용으로 복사 및 편집이 불가합니다.
서지정보
ㆍ발행기관 : 대한구강생물학회
ㆍ수록지정보 : International Journal of Oral Biology / 43권 / 2호
ㆍ저자명 : Jong-Jin Kil, Bong-Soo Park, Hae-Mi Kang, Su-Bin Yu, Dan-Bi Park, Jin-A Park, In-Ryoung Kim
목차
Introduction
Materials and Methods
Materials
Cell culture and luteolin treatment
MTT assay
Hoechst staining
Determination of apoptosis using flow cytometry
Western blot analysis
Invasion assay
Scratch migration assay
Quantitative PCR
Statistical analysis
Results
Evidence of apoptosis following luteolin treatment of OSCC
EMT pathway suppression and the inhibition of OSCC-related invasion and migration
Discussion
Conclusion
References
영어 초록
Oral squamous cell carcinoma (OSCC) is the most common type of oral malignancy. Numerous therapies have been proposed for its cure. Research is continually being conducted to develop new forms of treatment as current therapies are associated with numerous side-effects. Luteolin, a common dietary flavonoid, has been demonstrated to possess strong anti-cancer activity against various human cancer cell lines. Nevertheless, research into luteolin-based anticancer activity against oral cancer remains scarce. Thus, the objective of this study was to assess the effect of luteolin as an anti-cancer agent. After treatment with luteolin, Ca9-22 and CAL-27 oral cancer cells showed condensed nuclei and enhanced apoptotic rate with evidence of mitochondria-mediated apoptosis. Epithelial-mesenchymal transition (EMT) is closely related to tumor migration and invasion. Luteolin suppressed cancer cell invasion and migration in the current study. Elevated expression of E-cadherin, an adherens junction protein, was evident in both cell lines after luteolin treatment. Luteolin also significantly inhibited transcription factors (i.e., N-cadherin, Slug, Snail, Twist, and ZEB-1) that regulated expression of tumor suppressors such as E-cadherin based on Western blot analysis and quantitative PCR. Thus, luteolin could induce mitochondrial apoptosis and inhibit cancer cell invasion and migration by suppressing EMT-induced transcription factors.
참고 자료
없음
"International Journal of Oral Biology"의 다른 논문
더보기 (1/6)