한우의 수정란이식 효율성 향상을 위한 생체난포란 채취에 관한 연구

서지정보

ㆍ발행기관 : 한국수정란이식학회 ㆍ수록지정보 : Journal of Embryo Transfer / 32권 / 3호
ㆍ저자명 : 조상래, 강성식, 김의형, 이석동, 이명숙, 양병철

영어 초록

Commercial applications of OPU/IVP were to produce embryos and calves from high genetic cows.The aim of this present study was to compare the number of recovered oocytes and cultured in vitro produced embryos from Ovum Pick-up (OPU). OPU derived embryo production was carried out of oocytes by ultrasonographic guided follicular aspiration and then produced in vitro produced blastocysts by IVP culture system. In result, the rate of recovered oocytes was obtained 612 (57.2%) and 451(73.7) G1+G2 grade oocytes. No difference of recovered rate (51.162.1%) was seen in six donor. The rate of cleavage and blastocyst development were obtained 320 (70.9%) and 78 (24.4%) that was 3.30.4 cleaved embryo and 0.90.2 blastocysts per session. Cleavage rate of OPU oocytes in No. 6 donor was 90.6%, significantly (P0.05) higher than that in the other donors, However, blastocysts was similar (25.830.0%). In conclusion, limited numbers of OPU oocytes had competent development when cultured in SOF culture medium

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