쥐눈이콩-노루궁뎅이버섯 균사체 발효물의 생리활성

서지정보

ㆍ발행기관 : 한국식품영양학회 ㆍ수록지정보 : 한국식품영양학회지 / 30권 / 6호
ㆍ저자명 : 김훈, 신지영, 이아름, 황종현, 유광원

목차

서 론
재료 및 방법
1. 쥐눈이콩 발효물 조제를 위한 노루궁뎅이버섯 균사체종균배양
2. 고체발효에 의한 쥐눈이콩-노루궁뎅이버섯 균사체 발효물 제조
3. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물의 열수 및 주정추출물 조제
4. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물의 이소플라본 화합물 분석
5. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물 용매추출물의 항산화 활성
6. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물 용매추출물의 면역활성
7. 통계처리
결과 및 고찰
1. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물의 이소플라본 화합물 분석
2. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물 용매추출물의 항산화 활성
3. 쥐눈이콩-노루궁뎅이버섯 균사체 발효물 용매추출물의 마크로파지 활성
4. 쥐눈이콩 발효물 용매추출물의 Peyer’s patch를 경유한 장관면역 활성
요약 및 결론
감사의 글
References

영어 초록

To enhance the physiological activity of the Rhynchosia volubilis (RV), R. volubilis (RVHE-A) and R. volubilis-added herbal powder (RVHE-B) were fermented with a solid state culture of Hericium erinaceum mycelia (HE). The total isoflavone contents of the non-fermented RV-A (489.9 μg/g) and RV-B (571.1 μg/g) were remarkably increased in fermented RVHE-A (1,836.4 μg/g) and RVHE-B (1,276.7 μg/g). In particular, aglycone isoflavones such as daidzein and genistein were significantly higher in the RVHE-A than any other sample. When hot-water (HW) and EtOH extracts (E) were fractionated from the RV and RVHE, both extracts from the RVHE-A were higher than those from the RV-A in total polyphenol and flavonoid contents. However, the RVHE-B-HW showed a lower polyphenol and flavonoid content level than did RV-B-HW. RVHE-A-HW and -E also had more potent ABTS radical scavenging activity than any extract from the non-fermented RV and other ferments (RVHE-B). In the meanwhile, RVHE-A-HW potently stimulated the production of macrophage activation-related cytokines such as TNF-α, IL-6 and IL-12 (841.7±71.3 pg/mL, 3.9±0.1 ng/mL, 179.3±30.2 pg/mL) from peritoneal macrophage more than RV-A-HW (92.5±1.5 pg/mL, 0.1±0.0 ng/mL, 37.4±5.4 pg/mL) as well as RVHE-B-HW (557.0±21.3 pg/mL, 1.8±0.0 ng/mL, 90.0±10.0 pg/mL). However, all the EtOH extracts did not show significant activity. In addition, the RVHE-A-HW showed a significantly higher intestinal immune system modulating activity through Peyer’s patch and GM-CSF production than did any other extract from RV and RVHE-B. In conclusion, these results suggest that the fermented R. volubilis with H. erinaceum mycelia possesses a possible use as an industrial application as functional food or material.

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