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Isolation by Ion-Exchange Methods
Introduction
History
First reported in the late of 1930’s
First described in the late 1940’s
Techniques advantage for Using I.E chromatography
Convenient “ handle”
Achieve high level of purification
Easily scale-up
Low cost
*
Theory of Ion Exchange
Basis for Ion-Exchange separation
Reversible binding of charged molecular
Manipulating by solution pH
Dependent on the surface area of the stationary phase
Occur at the same time
G : charged functional groups
R : some support matrix
S : oppositely charged solute molecules
C : displacement of the counter-ion
RG-C+ + S+ ＲＧ－Ｓ＋＋Ｃ＋
RG＋C－ + S－ＲＧ＋Ｓ－＋Ｃ－

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