Analysis of Th1 vs Th2 gene expression
- 최초 등록일
- 2020.03.28
- 최종 저작일
- 2018.05
- 18페이지/
MS 파워포인트
- 가격 1,000원
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목차
1. Cytokine gene expression reflects T cell activation status
2. T helper cell differentiation and cytokine production
3. RT-PCR analysis of cytokine mRNA levels
4. What is PCR?
5. The exponential amplification of the gene in PCR.
6. The different steps in PCR.
7. What is RT-PCR?
8. RT-PCR step.
9. Materials
10. Methods- 1. Homogenizing samples
11. Methods – 2. RNA isolation
12. Methods – 3. check the RNA band
13. Methods – RT Reaction
14. Methods - PCR
본문내용
Cytokine gene expression reflects T cell activation status
Cytokines are small regulatory peptides of 10~20 kDa, which control the various aspects of immune cell function including cell growth, proliferation, differentiation and apoptosis.
Antigen stimulation of T cell receptor will generate intracellular signaling pathways to induce activation of transcription factors. which, through binding to the cytokine gene promoters will induce transcription of specific cytokine genes.
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