TRAP Staining-Protocol

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TRAP Assay Leucocyte Acid phosphate stain sigma, cat No. 387-A

Reagent Preparation samle no: (+1)
Volume(ul) amounts/sample 18
Reagent 1, fast Garnet GBC 10 33.33333333 600 ul
Reagent 2 Sodicum Nitrate 10 33.33333333 600 ul
Mix together and let it stand 2min in dark 0
Reagent 3 DDW 900 3000 54000 ul
Reagent 4 Naphthol AS-BI phosphate sol. 10 33.33333333 600 ul
Reagent 5 Acetate sol. 40 133.3333333 2400 ul
Reagent 6 Tartrate sol. 20 66.66666667 1200 ul
Total amount 59400 ul
Fixation & Permeation
Add 300ul of 3.7% Formalin RT 30min Junsei
Add 200ul 0.1% Triton X- 100, RT 5 min
Wash Twice with DDW

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