[연세대 10/10 A+] 공학생물학및실험(2) 1주차 PCR 결과레포트

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"[연세대 10/10 A+] 공학생물학및실험(2) 1주차 PCR 결과레포트"에 대한 내용입니다.

목차

1. Purpose
2. Results
3. Discussion
4. Further Study

본문내용

Purpose:

The purpose of the experiment is to amplify a small sample DNA sufficiently into a single PCR product to ensure all sizes of DNA can be amplified to completion through the same process.

< 중 략 >

Figure 1 shows the DNA band of PCR sample after agarose gel electrophoresis along with two markers with given base pairs. The size of PCR products are conventionally calculated using the distance travelled on agarose gel after gel electrophoresis. Since only a digital copy of result image was given from the experiment, the tool Image Viewer on MATLAB was used to measure the distance travelled on gel in an arbitrary unit as shown in Figure 2. Figure 3 shows the calibration curve of distance travelled on agarose gel against the logarithmic value of DNA size in base pairs to the base of 10. The size of DNA fragments in base pairs is converted into logarithmic value to the base of 10 to ensure a linear graph is plotted instead of a polynomial graph.

참고 자료

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Cha, Rita, and William Thilly. “Specificity, Efficiency, and Fidelity of PCR”. genome.cshlp.org, genome.cshlp.org/content/3/3/S18.full.pdf. Accessed 12 Sept 2021.
“Experiment 3: Polymerase Chain Reaction (PCR)”. Southeastern, southeastern.edu/Academics/Faculty/jtemple/486/experiment%203.pdf. Accessed 11 Sept 2021.
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