BMDM isolation and culture
- 최초 등록일
- 2022.02.25
- 최종 저작일
- 2021.08
- 5페이지/ 한컴오피스
- 가격 1,500원
소개글
"BMDM isolation and culture"에 대한 내용입니다.
목차
1. Title
2. Purpose
3. Materials
4. Methods
5. Results
6. Discussion
(1) bone isolation
(2) cell counting
본문내용
4. Purpose : mouse의 다리뼈에서 골수 세포를 채취해 대식세포로 분화시키기
5. Materials
-fetal bovine serum, DMEM medium supplemented with 20% (vol/vol) fetal calf serum, LCM (L929 cell conditional media, 50 μM 2-mercaptoethanol), 20% FBS media, L929 cell conditional media, Tripcine-EDTA, 100mM 2-mercaptoethanol, 14.3M (Mol concentration of 2-mercaptoethanol), auto DIW, mouse, T.B
6. Methods
mouse : C57BL6 mice
- DMEM media
- Fetal Bovine Serum
- Media : DMEM medium supplemented with 20% (vol/vol) fetal calf serum, LCM (L929 cell conditional media, 50 μM 2-mercaptoethanol)
<중 략>
[ Day 0 ]
1. Bone isolation
2. Centrifuge (1300 rpm, 5 min)
3. ACK solution 1mL add and reaction for 2 min at RT
4.1X PBS 10ml add and centrifuge
5. 1XPBS wash (repeat 2)
6. Media 10 ml add
7. cell strainer and cell counting
8. cell seeding (5X10^6 cells/100Φ bacteria dish)
[ Day 1 ]
1. Collect non-adherent cells by gently pipetting
2. Centrifuge 1300 rpm, 5min
참고 자료
없음