식품 생화학 실험 real time qPCR과정과 결과값
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- 2024.04.25
- 최종 저작일
- 2023.05
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"식품 생화학 실험 real time qPCR과정과 결과값"에 대한 내용입니다.목차
1. Title2. Date
3. Objective
4. Introduction
5. Materials & Method
6. Results
7. Discussion
8. Reference
본문내용
1. Title : RT-qPCR2. Date : 2023.05.31
3. Objective: 합성한 cDNA로 Real-time qPCR을 수행한다.
4. Introduction
(1) GADPH
GAPDH 유전자는 glyceraldehyde-3-phosphate 탈수소효소 단백질 계열의 구성원을 암호화한다. 암호화된 단백질은 기계적으로 구별되는 능력을 기반으로 월광 단백질로 확인되었다. 이 유전자의 산물은 무기 인산염과 nicotinamide adenine dinucleotide (NAD)의 존재 하에서 glyceraldehyde- 3-phosphate의 가역적 산화적 인산화인 탄수화물 대사에서 중요한 에너지 생성 단계를 촉매한다.
참고 자료
Pubmed, Kavleen Sikand, Jagjit Singh, Jey Sabith Ebron, Girish C. Shukla, Housekeeping Gene Selection Advisory: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and β-Actin Are Targets of miR-644a, 2012.10.16.thermofisher.com, SYBR Green , 2023.05.30.,
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enzolifesciences.com, RT-qPCR과 RT-PCR의 차이점, 2023.05.31.,
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