[유전공학]유전자 조작과 형질전환

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유전자 조작과 형질전환중 유전자의 정의, Central dogma, cloning의 목적!!

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목차

What is Gene?
Structure of gene
Central Dogma
Cloning
Recombinant DNA
Genome & cDNA cloning
Selection of cloned gene
Application of gene clone

본문내용

What is Gene?
Gene :
regions of chromosomal DNA encoding proteins
a length of DNA which codes for a particular protein,
or in certain cases a functional or structural RNA molecule
Regulatory gene
Structural gene
Structure of gene
Gene. ORF
Codon. Encoding. Initiation codon. Termination (nonsense codon)
Central Dogma
DNA-RNA-Protein
Unidirectional processing
Replication, Transcription, Termination
Cloning
Clone : identical
Cloning : copy
-The process of producing identical copies (clones) of a gene
Recombinant DNA
Recombinant DNA :
DNA molecules that have been created by combining DNA
more than one source.
Plasmid. Restriction NZ. Ligation. Bacterial transformation
Genome & cDNA cloning
Tissue, Cell
DNA
extraction
mRNA
extraction
Genome
cloning
Gene
expression
DNA
sequencing
cDNA
synthesis
Recombinant
protein
Subcloning
cDNA
cloning

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