stem cell tracking with nanoparticle
- 최초 등록일
- 2007.02.02
- 최종 저작일
- 2006.12
- 22페이지/
MS 파워포인트
- 가격 5,000원
소개글
줄기세포의 분화를 확인하는데 나노입자를 사용하면 안정적이며 효과적이라는 실험논문을 보고 파워포인트 발표자료로 정리한것입니다.
목차
Abstract
Determination of cellular FITC-MSN uptake by flow cytometry
Determination of cellular FITC-MSN uptake by microscopic studies
Immunophenotyping of hMSCs
본문내용
☀ Tracking the distribution of stem cells is crucial to their therapeutic use.
☀ Cellular labeling approach with a novel vector composed of mesoporous silica
nanoparticles (MSNs) conjugated with fluorescein isothiocyanate in human bone
marrowmesenchymal stem cells and 3T3-L1 cells.
☀ FITC-MSNs were efficiently internalized into mesenchymal stem cells and 3T3-L1
cells even in short-term incubation.
☀ The process displayed a time- and concentration-dependent manner and was
dependent on clathrin-mediated endocytosis.
⇒ advantages of biocompatibility, durability, and higher efficiency in
internalization suit MSNs to be a better vector for stem cell tracking than
others currently used.
☀ The internalization of FITC-MSNs did not affect the cell viability,proliferation,
immunophenotype, and differentiation potential of mesenchymal stem cells, and
3T3-L1 cells.
☀ FITC-MSNs could escape from endolysosomal vesicles and were retained
the architectonic integrity after internalization.
참고 자료
논문제목 : Highly efficient cellular labeling of mesoporous nanoparticles in human mesenchymal stem cells: implication for stem cell tracking.
출처 : The FASEB Journal express article 10.1096/fj.05-4288fje. Published online October 17, 2005.
