Dental pulp mesenchymal stem cells

소개글

Dental Pulp 에서 Stem cell isolation 과 배양법

목차

Multipltent stem cells in dental pulp
1.1. Introduction
1.2. Preparation of media and reagents
1.2.1. Culture media
1.2.1.1. Mesenchymal Stem cell medium (MSC medium)
1.2.1.2. Odontogenic Differentiation medium
1.2.1.3. Adipogenic Differentiation medium
1.2.1.4. Neural Differentiation medium
1.2.2. Solutions for tissue digestion
1.2.2.1. Collagenase solution
1.2.2.2. Dispase solution
1.2.3. Freezing medium]
1.2.3.1. Freezing medium I
1.2.3.2. Freezing medium II
1.3. Dentaled protocols for culture
1.3.1. Safety Precautions
1.3.2. Tissue Handling
1.3.3. Isolation of dental pulp tissue
Protocol 1.1. Isolation of dental pulp tissue

본문내용

MULTIPOTENT STEM CELLS IN DENTAL PULP

1.1. INTRODUCTION
The crowns of human teeth consist of enamel, dentin, and dental pulp tissue. During tooth growth and development, ameloblasts form enamel and odontoblasts generate primary dentin. After tooth eruption, ameloblasts disappear from the surface of the enamel; consequently, enamel formation ceases to occur naturally in vivo. In contrast, odontoblast, along the inner surface of the dentin inside the pulp chamber, continue to deposit dentin matrix to form secondary dentin throughout life. In addition to secondary dentin, odontoblasts can form tertiary (reactionary/reparative) dentin in response to several stimuli, such as mechanical, chemical, and/or bacterial stimulation.

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