향상된 세포 파종 효율을 위한 양극화된 단백질 멤브레인
- 최초 등록일
- 2008.10.22
- 최종 저작일
- 2008.10
- 34페이지/
MS 파워포인트
- 가격 1,000원
소개글
조직공학용 스케폴드로써 세포의 파종 효율을 높이고, 세포의 분배, 침투, 부착등의 기능을 높이기 위해, 기공이 넓은 일반적 고분자 직물에 단백질 멤브레인으로 계층적으로 형성시켜 상부는 엉성하고, 하부는 촘촘한 기공구조 도입한 연구입니다.
목차
Polarized Protein Membrane for High Cell Seeding Efficiency
1. INTRODUCTION
1-1. Schematic representation of the principle
1-2. Gradient Porosity Networks Scaffold
1-3. Objective
2. MATERIALS AND METHODS
2-1. Materials
2-2. Instrumentation
3. EXPERIMENTAL PROCEDURES
3-1. Preparation of Polymer Surfaces
3-2. Collagen Immobilization Method
3-3. Fibrin Immobilization Method
3-4. Principle of Gradient Porosity
3-5. Creation of the Polarized Membrane
3-6. Cell Culture
3-7. 3D Cell Seeding and Determination of Cell
3-8. Seeding Efficiency
3-9. 3T3 cells
3-10. Microscopy
3-11. Proliferation Study
4. RESULTS
4-1. PET knitted fabric before protein immobilization
4-2. Effect of hydrolysis process
4-3. PET knitted fabric after protein immobilization
4-4. Cell Seeding Efficiency
4-5. Cell distribution depend on direction of gradient scaffold
4-6. Statistical Data of Distribution in Polarized Membrane
4-7. Cell Proliferation
5. DISCUSSION
5-1. Gradient Porosity Networks
5-2. The benefit of this research
6. CONCLUSION
본문내용
Objective
• Development of a gradient porosity scaffold similar
to a filter made from collagen or fibrin
• Preparation of large open pores (20–50 um) at the scaffold surface to facilitate cell penetration, and dense pores (<1 um) at the scaffold bottom prevent cells leakage
• Evaluation of cell seeding efficiency, cell distribution and proliferation through the scaffold resulting in quantitative seeding in three dimensions
<중 략>
CONCLUSION
• A polarized membrane showed efficient cell seeding from results of SEM, histology, cell seeding experiments and proliferation studies.
• This study has a simple and reliable procedure, whereby scaffold materials can be processed into gradient membranes with good cell adhesion and seeding capabilities.
• The scaffold allows not only effective seeding but also the fast proliferation of the cells.
참고 자료
Polarized Protein Membrane for High Cell Seeding Efficiency, Bjo¨rn Atthoff, Cecilia Aulin, Catharina Adelo¨ w, Jo¨ ns Hilborn, Journal of Biomedical Materials Research Part B: Applied Biomaterials 83B: 472–480 (2007)
