Then determine the background radiation{ dot N_0 without the preparation and substract it from the measured ... After determining the background radiation count { dot N_0 without the preparation, count the impulses ... , we obtain a straight line of slope -2.
, slide마다 staining된 것을 확인하면 바로 PBS 통에 넣어 DAB을 씻어내 background (non-specific staining)를 줄인다 Stable DAB ... Incubate in Vector blue Alkaline phosphatase substract kit (AK-5000) with PBS 30 min. ... Immunohistochemistry (Double staining) * Paraformaldehyde로 고정된 slide 준비 Slide의 sample이 마르지 않도록 다음단계의