PhosphoproteinEnrichedinAstrocytes15(PEA15)가 과발현하는 형질전환 돼지의 생산

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : 이휘철, 김현미, 이승훈, 오건봉, 정학재, 양병철, 김경운, 이풍연, 박진기, 장원경

목차

ABSTRACT 서론 재료 및 방법 미세 주입용 유전자 준비 Micropipets 제작 및 유전자 미세 주입 외과적 방법에 의한 수정란 채란 및 이식 RT-PCR RFLP 및 Real-time RT-PCR Southern Blot Hybridization Western Blotting Fluorescence In situ Hybridization (FISH) 결과 및 고찰 mPEA15의 과발현 및 EGFP 발현의 확인 인용문헌

영어 초록

The overexpression of Phosphoprotein Enriched in Astrocytes (PEA15) gene is commonly found in human diabetic patients. The overexpression of this gene in skeletal muscle and fat tissues have been reported to cause insulin resistance, thereby impairing insulin stimulated glucose uptake. We introduced a gene of mouse PEA15 (mPEA15) and enhanced green fluorescent protein (EGFP) into fertilized one cell pig zygotes using microinjection, and produced a piglet that showed overexpression of mPEA15 in the muscle tissues and expression of EGFP in the ear tissues and hooves. RT-PCR RFLP, southern blot and FISH analysis showed that the tissues carried the transgene. Real-time RT-PCR and western blots demonstrated that PEA15 gene was overexpressed in the various tissues and muscle tissues, respectively. These facts suggest that expression vector system is normally expressed in the trnasgenic (TG) pigs. To use as animal diseases model for type 2 diabetes, further study is necessary to confirm whether diabetes occur in these TG pigs, especially insulin resistance.

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