공여세포 처리 조건이 형질전환 복제돼지 생산에 미치는 영향

서지정보

ㆍ발행기관 : 한국동물번식학회 ㆍ수록지정보 : Reproductive & developmental biology / 35권 / 3호
ㆍ저자명 : 권대진, 곽태욱, 오건봉, 김동훈, 양병철, 임기순, 김진회, 박진기, 황성수

목차

ABSTRACT 서론 재료 및 방법 화학 조성물 미성숙 난자의 체외성숙 공여세포 준비 핵이식 과정 형질전환 복제란 이식 및 임신 확인 통계적 분석 결과 공여세포에 적합한 융합조건 확립 이식된 형질전환 복제란의 숫자가 임신율 및 분만율에 미치는 영향 분석 공여세포 배양조건에 따른 임신율 및 분만율 분석 고찰 인용문헌

영어 초록

This study was conducted to investigate the effects of donor cell treatments on the production of transgenic cloned piglets. Ear fibroblast cell obtained from NIH MHC Inbred minipig was used as control. The GalT knock-out/CD46 knock-in (GalT/CD46) transgenic cell lines were established and used as donor cells. The reconstructed GalT/CD46 embryos were surgically transferred into oviduct of naturally cycling surrogate sows (Landrace × Yorkshire) on the second day of standing estrus. Unlike control (1.2 kV/cm,, 75.4%), the fusion rate of the GalT/CD46 donor cells was significantly higher in 1.5 kV/cm, (84.5%) than that of 1.25 kV/cm, (20.2%) (p<0.01). When the number of the transferred embryos were more than 129, the pregnancy and delivery rates were increased to 13/20 (65%) and 5/20 (25%) compared to less than 100 group [1/6 (16.7%) and 0/6 (0%)], respectively. To analyze the effect of donor cell culture condition on pregnancy and delivery rates, the GalT/CD46 donor cells were cultured with DMEM or serum reduced medium. In serum reduced medium group, the pregnancy and delivery rates were improved to 8/12 (66.7%) and 5/12 (41.7%) compared to DMEM group [3/7 (42.9%) and 0/7 (0%)], respectively. In conclusion, it can be postulated that an appropriate fusion condition and culture system is essential factors to increase the efficiency of the production of transgenic cloned piglets.

참고 자료

없음