한우에서 Myopalladin (MYPN) 유전자의 A1795G SNP의 다형성 및 도체형질 관련성 분석
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- 2023.04.05
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서지정보
ㆍ발행기관 : 강원대학교 동물자원공동연구소
ㆍ수록지정보 : 동물자원연구 / 22권 / 2호
ㆍ저자명 : 홍민욱, 김훈, 박새롬, 이승규, 이영섭, 김진우, 김기범, 이선영, 송영한, 이성진
목차
ABSTRACT Ι. 서론 Ⅱ. 재료 및 방법 1. 공시재료 2. 시료의 DNA 동일성 검정 3. Genomic DNA의 분리 및 정제 4. MYPN 유전자의 primer 설계 및 합성 5. MYPN 유전자의 PCR 증폭 6. PCR-RFLP 분석에 의한 MYPN A1795G SNP의 유전자형 확인 7. 통계분석 Ⅲ. 결과 및 고찰 Ⅳ. 요약 Ⅴ. 인용문헌
영어 초록
Myopalladin (MYPN) is an important expression gene associated with regulation of Z-line structure in muscle and maintains sarcomeric integrity. In this study, we investigated the association between MYPN A1795G SNP (single nucleotide polymorphism) and carcass traits (LMA, longissimus muscle area; CW, carcass weight; BF, backfat thickness; MS, marbling score) in Korean cattle. The MYPN A1795G SNP was genotyped in 212 steers and analyzed the associations with carcass traits by PCR-RFLP (Restriction fragment length polymorphism) method. The allele frequencies were 0.566 for G allele and 0.434 for A allele. And the genotype frequencies of GG, GA, and AA genotype were 32.1%, 49%, and 18.9%, respectively. Association analysis indicated that the A1795G SNP of MYPN gene showed a significant association with LMA (p<0.05). The steers with GG genotype had higher LMA than those with the genotypes AA. But no significant associations were observed in other carcass traits (CW, BF, MS). The steers with the GG genotype showed higher CW and BF than those with the genotypes AA and GA. These results suggest that the A1795G SNP of the MYPN gene is associated with LMA and may be useful for candidate marker-assisted selection to increase the levels of LMA in Korean cattle.
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